Results
| PMID | 10050665 |
| Gene Name | MANEA |
| Condition | Endometriosis |
| Association |
Associated |
| Sex | Female |
| Associated genes | ENDO-I |
| Other associated phenotypes |
Endometriosis, angiogenesis, altered immune response |
|
Mol Hum Reprod. 1999 Jan;5(1):71-8. Piva, M| Sharpe-Timms, K L Department of Obstetrics and Gynecology, The University of Missouri, Columbia 65212, USA. A unique glycoprotein, called endometriosis protein-I (ENDO-I), has previously been shown to be synthesized and secreted by rat and human endometriotic explants. Furthermore, the N-terminal amino acid sequence analysis showed that ENDO-I shares homology with haptoglobin. The present study was designed to determine this sequence of ENDO-I cDNA from human peritoneal endometriotic lesions and to determine the relative expression of the ENDO-I gene in several human tissues. Poly A-enriched RNA was isolated and reverse-transcribed. To determine the sequence of ENDO-I cDNA, a polymerase chain reaction was performed on cDNA from human endometriotic lesions and a gene-specific primer based on the human haptoglobin sequence. A similar procedure was followed to assess the relative expression of the ENDO-I gene in various tissues. Glyceraldehyde-3-phosphate-dehydrogenase was used as an internal control. ENDO-I gene expression was quantified by densitometry. Sequence analysis of ENDO-I cDNA identified 873 nucleotides that displayed 99.4% homology with the human haptoglobin beta-chain. Relative expression of ENDO-I mRNA by peritoneal endometriotic lesions was 19-fold greater than peritoneum, 28-fold greater than endometrioma and 37-fold greater than eutopic endometrium (P<0.01). Haptoglobin-like ENDO-I may be associated with localized angiogenesis and altered immune response involved with the aetiology/pathophysiology of endometriosis. Mesh Terms: Adolescent| Adult| Animals| Base Sequence| Case-Control Studies| Endometriosis/*genetics| Endometrium/metabolism/pathology| Female| Gene Expression Regulation| Glycoproteins/*genetics| Haptoglobins/*genetics| Humans| Liver/physiology| Middle |
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