Results
| PMID | 10593388 |
| Gene Name | CYP2B6 |
| Condition | Endometriosis |
| Association |
Associated |
| Population size | 105 |
| Population details | 105 women of reproductive age with normal menstrual cycles underwent endometrial biopsy laparotomy or laparoscopy, and examination of their tissue revealed endometriosis, adenomyosis, and/or leiomyomas |
| Sex | Female |
| Infertility type | Female infertility |
| Other associated phenotypes |
Endometriosis, adenomyosis, and/or leiomyomas |
|
Fertil Steril. 1999 Dec;72(6):1100-6. Kitawaki, J| Kusuki, I| Koshiba, H| Tsukamoto, K| Fushiki, S| Honjo, H Department of Obstetrics and Gynecology, Kyoto Prefectural University of Medicine, Japan. kitawaki@koto.kpu-m.ac.jp OBJECTIVE: To evaluate the clinical usefulness of examining endometrial biopsy specimens for aromatase cytochrome P-450 as a diagnostic test for endometriosis. DESIGN: Retrospective, case-controlled study. SETTING: Department of Obstetrics and Gynecology, Kyoto Prefectural University of Medicine, Kyoto, Japan. PATIENT(S): One hundred five women of reproductive age with normal menstrual cycles underwent endometrial biopsy laparotomy or laparoscopy, and examination of their tissue revealed endometriosis, adenomyosis, and/or leiomyomas. Patients who had cervical carcinoma in situ but no other gynecologic disease were considered to be disease-free. INTERVENTION(S): Endometrial biopsy specimens were collected. MAIN OUTCOME MEASURE(S): The expression of aromatase cytochrome P-450 was examined by reverse transcription-polymerase chain reaction and immunohistochemical analysis. The distribution and intensity of the immunostaining was assessed using a semiquantitative index designed H-score. RESULT(S): Immunostaining for aromatase cytochrome P-450 was detected in biopsy specimens obtained from patients with endometriosis, adenomyosis, and/or leiomyomas but not in specimens obtained from disease-free patients (H-score <20), with a sensitivity and specificity of 91% and 100%, respectively. CONCLUSION(S): The expression of aromatase cytochrome P-450 in biopsy specimens of eutopic endometrium distinguishes between disease-free women and women with endometriosis, adenomyosis, and/or leiomyomas. This technique can be used at outpatient infertility clinics as an initial screening procedure to rule out the presence of estrogen-dependent disease. Mesh Terms: Adult| Aromatase/*analysis| Biopsy| Endometriosis/diagnosis/*enzymology| Endometrium/*enzymology/pathology| Female| Humans| Immunohistochemistry| Middle Aged| Predictive Value of Tests| Retrospective Studies|DA 1999/12/11 00:01 |
|