Results
| PMID | 12372463 |
| Gene Name | CYP2B6 |
| Condition | Endometriosis (Pelvic) |
| Association |
Associated |
| Population size | 60 |
| Population details | 60 subjects of reproductive age undergoing laparoscopy for subfertility exploration, pain assessment, or sterilization |
| Sex | Female |
| Infertility type | Female infertility |
| Associated genes | P450 |
| Other associated phenotypes |
Pelvic endometriosis, Infertility |
|
Fertil Steril. 2002 Oct;78(4):825-9. Dheenadayalu, Kavitha| Mak, Ian| Gordts, Stephan| Campo, Rudi| Higham, Jenny| Puttemans, Patrick| White, John| Christian, Mark| Fusi, Luca| Brosens, Jan Department of Obstetrics and Gynaecology, Ealing Hospital, London, United Kingdom. OBJECTIVE: To determine whether expression of aromatase P450 mRNA in eutopic endometrium is predictive of the presence of pelvic endometriosis. DESIGN: A prospective, multicenter, observational study. SETTING: Four tertiary centers for reproductive medicine. PATIENT(S): Sixty subjects of reproductive age undergoing laparoscopy for subfertility exploration, pain assessment, or sterilization. INTERVENTION(S): Endometrial biopsy at time of laparoscopy. MAIN OUTCOME MEASURE(S): The expression of aromatase P450 mRNA in endometrial specimens was determined by single-tube reverse transcription-polymerase chain reaction (RT-PCR). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA was amplified in parallel to exclude amplification failure. RESULT(S): The RT-PCR amplification was successful in 56 of the 60 biopsies (93%). Pelvic endometriosis was diagnosed in 34 patients (61%) and was strongly associated with aromatase P450 mRNA expression in eutopic endometrium. As a diagnostic marker for endometriosis, aromatase P450 mRNA expression yielded a sensitivity of 82%, a specificity of 59%, a positive predictive value of 76%, and a negative predictive value of 67%. If additional uterine pathology was taken in account, the sensitivity increased to 84%, the specificity to 72%, the positive predictive value to 87%, but the negative predictive value remained unchanged (67%). CONCLUSION(S): Although endometrial aromatase P450 gene expression is highly predictive of the presence of pelvic disease, the relative high incidence of false-negative results and lack of specificity is likely to impair clinical application. Mesh Terms: Adult| Aromatase/*genetics| Biomarkers/*analysis| Biopsy| Endometriosis/*enzymology| Endometrium/*enzymology| False Negative Reactions| Female| Glyceraldehyde-3-Phosphate Dehydrogenases/genetics| Humans| Laparoscopy| Middle Aged| Prospective S |
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