Results
| PMID | 12620496 |
| Gene Name | CXCL5 |
| Condition | Endometriosis |
| Association |
Associated |
| Population size | 27 |
| Population details | 27 (18 women with endometriosis, 9 women without endometriosis) |
| Sex | Female |
| Associated genes | ENA-78 |
| Other associated phenotypes |
Endometriosis |
|
Fertil Steril. 2003 Mar;79 Suppl 1:815-20. Mueller, Michael D| Mazzucchelli, Luca| Buri, Caroline| Lebovic, Dan I| Dreher, Ekkehard| Taylor, Robert N Department of Obstetrics and Gynecology, Inselspital, University of Bern, Bern, Switzerland. michel.mueller@insel.ch OBJECTIVE: To investigate the presence of epithelial neutrophil-activating peptide 78 (ENA-78) in peritoneal fluid of women with and without endometriosis and to identify the cells that produce this inflammatory protein. DESIGN: Case-control study. SETTING: University hospital. PATIENT(S): Eighteen women with and 9 women without endometriosis. MAIN OUTCOME MEASURE(S): ENA-78 protein and mRNA levels were compared among women with and without endometriosis in samples of peritoneal fluid, samples of endometriotic lesions obtained by biopsy during laparoscopy, and peritoneal macrophages. Enzyme-linked immunosorbent assay, reverse transcription polymerase chain reaction, and in situ hybridization methods were used. Secretion of ENA-78 protein by interleukin-1beta-stimulated endometriotic stromal cells and in the media of lipopolysaccharide-stimulated peritoneal macrophages were compared to that in unstimulated cell cultures. RESULT(S): Peritoneal fluid concentrations of ENA-78 were significantly higher in affected women than in controls. Ectopic epithelial and stromal cells and peritoneal macrophages express ENA-78 messenger RNA. Interleukin-1beta stimulation of stromal cell cultures resulted in a 23-fold increase in ENA-78 concentration, and lipopolysaccharide stimulation of peritoneal macrophages increased concentrations by 8-fold. CONCLUSION(S): Levels of ENA-78 are elevated in the peritoneal fluid of women with endometriosis. Ectopic glandular cells, ectopic stromal cells, and peritoneal macrophages express this inflammatory chemokine. Epithelial neutrophil-activating peptide 78 may play an important role in the pathogenesis of endometriosis. Mesh Terms: Ascitic Fluid/*metabolism| Case-Control Studies| Chemokine CXCL5| *Chemokines, CXC| Endometriosis/*metabolism| Female| Humans| In Situ Hybridization| Interleukin-1/pharmacology| Interleukin-8/*analogs & derivatives/*biosynthesis/genetics| Lipopo |
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