Results
| PMID | 15705625 |
| Gene Name | IL1R2 |
| Condition | Endometriosis |
| Association |
Associated |
| Sex | Female |
| Associated genes | IL-1RII |
| Other associated phenotypes |
Endometriosis |
|
Hum Reprod. 2005 May;20(5):1177-84. Epub 2005 Feb 10. Bellehumeur, C| Collette, T| Maheux, R| Mailloux, J| Villeneuve, M| Akoum, A Centre de Recherche, Hopital Saint-Francois d'Assise, Centre Hospitalier Universitaire de Quebec, Canada. Numerous functional changes were observed in the intrauterine endometrial tissue of women with endometriosis. Our previous studies revealed a marked decrease in the expression of interleukin-1 receptor type 2 (IL-1RII), a decoy receptor known for its ability to buffer IL-1 effects. The aim of the present study was to assess whether post-translational mechanisms such as proteolysis may contribute to the down-regulation of IL-1RII levels. Our data showed that soluble IL-1RII (sIL-1RII) concentrations released by freshly cultured endometrial tissue were significantly lower in women with endometriosis than in normal women (P < 0.01) and further revealed a statistically significant correlation between increased proteolysis and decreased sIL-1RII levels (P < 0.05; r = -0.47). (125)I-labelled soluble recombinant human IL-1RII ([(125)I]srhIL-1RII) was significantly more degraded in culture supernatant of tissues from women with endometriosis compared to normal women (P < 0.05), and natural tissue inhibitor of matrix metalloproteinase (TIMP)-1 inhibited [(125)I]srhIL-1RII degradation. Incubation of srhIL-1RII with active rhMMP-9 resulted in a dose-dependent degradation of srhIL-1RII as analysed by western blotting. Dual immunofluorescence showed an increased immunostaining for matrix metalloproteinase-9 in situ in the endometrial tissue of women with endometriosis compared to normal women and a decreased immunostaining for IL-1RII. The present study showed a reduced release of sIL-1RII by the endometrial tissue of women with endometriosis and revealed a proteolytic post-translational mechanism which may be involved in the down-regulation of IL-1RII levels. This may enhance IL-1-mediated activation of endometrial cells and contribute to the local immuno-inflammatory process observed in endometriosis patients. Mesh Terms: Adult| Endometriosis/*metabolism/pathology| Endometrium/*metabolism/pathology| Female| Humans| Iodine Radioisotopes| Matrix Metalloproteinase 9/metabolism| Matrix Metalloproteinase Inhibitors| Protein Processing, Post-Translational| Receptors, In |
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