Results
| PMID | 16096323 |
| Gene Name | F2RL1 |
| Condition | Endometriosis |
| Association |
Associated |
| Sex | Female |
| Associated genes | IL-6, IL-8, PAR2 |
| Other associated phenotypes |
Endometriosis |
|
Hum Reprod. 2005 Dec;20(12):3547-53. Epub 2005 Aug 11. Hirota, Yasushi| Osuga, Yutaka| Hirata, Tetsuya| Harada, Miyuki| Morimoto, Chieko| Yoshino, Osamu| Koga, Kaori| Yano, Tetsu| Tsutsumi, Osamu| Taketani, Yuji Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo, 7-3-1, Hongo, Tokyo, 113-8655, Japan. BACKGROUND: Inflammation has been proposed to play essential roles in the pathophysiology of endometriosis, in which neutrophils and mast cells have been suggested to be involved. We studied whether the protease-activated receptor 2 (PAR2), which is activated by enzymes from neutrophils and mast cells, in endometriotic stromal cells (ESC) has any implication in the development of the disease. METHODS: Cultured ESC were stimulated with various concentrations of a specific PAR2 agonist peptide. Proliferating activity of the cells was determined using immunostaining of proliferating cell nuclear antigen (a cell proliferation marker), 5-bromo-2'-deoxyuridine incorporation into DNA and cell count. The concentrations of interleukin (IL)-6 and IL-8 were measured using specific enzyme-linked immunosorbent assay kits. The phosphorylation of three mitogen-activated protein kinases (MAPK), i.e. p38 MAPK, p42/44 MAPK and stress-activated protein Kinase/c-jun N terminal Kinase, in ESC was examined with Western blot analysis. RESULTS: Activation of PAR2 stimulated the proliferation of ESC and the secretion of IL-6 and IL-8 from ESC in a dose-dependent manner. Activation of PAR2 stimulated the phosphorylation of all three MAPK, and inhibitors of each MAPK suppressed the PAR2 activation-induced proliferation of ESC. CONCLUSIONS: The activation of PAR2 in ESC may be involved in the pathophysiology of endometriosis by inducing the growth and inflammation of endometriotic lesions. Mesh Terms: Blotting, Western| Bromodeoxyuridine/pharmacology| Cell Count| Cell Proliferation| DNA/chemistry/metabolism| Dose-Response Relationship, Drug| Endometriosis/pathology| Endometrium/*pathology| Enzyme Activation| Enzyme Inhibitors/pharmacology| En |
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