Results
| PMID | 17295901 |
| Gene Name | PTGS2 |
| Condition | Endometriosis |
| Association |
Associated |
| Population size | 28 |
| Population details | 28 patients with endometriosis |
| Sex | Female |
| Associated genes | PGES, COX-2 |
| Other associated phenotypes |
Endometriosis |
|
Am J Reprod Immunol. 2007 Mar;57(3):218-26. Chishima, Fumihisa| Hayakawa, Satoshi| Yamamoto, Tatsuo| Sugitani, Masahiko| Karasaki-Suzuki, Miki| Sugita, Kenji| Nemoto, Norimichi Department of Obstetrics and Gynecology, Nihon University School of Medicine, 30-1 Oyaguchi-kamimachi, Itabashi-ku, Tokyo 173-8610, Japan. fchishi@med.nihon-u.ac.jp PROBLEM: Recently, an inducible microsomal human prostaglandin E synthase (mPGES) was identified. This enzyme converts the cyclooxygenase (COX) product, prostaglandin (PG) H(2), to PGE(2), an eicosanoid linked to carcinogenesis. Although elevated levels of PGE(2) have been observed in many tumor types including colorectal adenomas and cancers, its role in the pathophysiology of endometriosis is unknown. We previously reported increased expression of COX-2 messenger RNA (mRNA) in local lesions of endometriosis. To further elucidate the mechanism responsible for the elevated levels of PGE(2) in endometriosis, we examined the expression levels of mPGES. METHOD OF STUDY: Samples were obtained from 28 patients, fixed in formalin, and embedded in paraffin for immunohistochemical analysis. We examined the expression of mPGES mRNA in seven cases by reverse transcriptase-polymerase chain reaction using total RNA extracted from frozen samples. RESULTS: Immunohistochemistry revealed increased mPGES immunoreactivity in endometriosis samples compared with eutopic endometria. Microsomal PGES immunoreactivity was observed in both epithelial cells and stromal or inflammatory cells of endometriosis. Increased expression of mPGES-1 mRNA was detected in most of the endometriosis samples. CONCLUSION: Our results suggest that expression of mPGES in addition to COX-2 plays a role in increasing PGE(2) production in endometriosis. Mesh Terms: Adult| Cyclooxygenase 2/genetics/metabolism| Endometriosis/*enzymology| Endometrium/enzymology/metabolism| Epithelial Cells/enzymology/metabolism| Female| Gene Expression| Humans| Immunohistochemistry| Intramolecular Oxidoreductases/genetics/*met |
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