Results
| PMID | 17531990 |
| Gene Name | HLA-DRB1 |
| Condition | Endometriosis |
| Association |
Associated |
| Population size | 129 |
| Population details | 129 (64 Japanese endometriosis patients, 65 women with other laparoscopic diagnoses) |
| Sex | Female |
| Infertility type | Female infertility |
| Associated genes | HLA-ABC, HLA-DR, IFN-gamma |
| Other associated phenotypes |
Endometriosis |
|
Fertil Steril. 2008 Jan;89(1):52-9. Epub 2007 May 25. Yamamoto, Yorito| Maeda, Nagamasa| Izumiya, Chiaki| Kusume, Tomoaki| Oguri, Hiroyoshi| Kawashima, Masaaki| Hayashi, Kazutoshi| Nomura, Aki| Yamashita, Chika| Fukaya, Takao Department of Obstetrics and Gynecology, Kochi Medical School, Kohasu, Oko, Nankoku, Kochi, Japan. yoritoy@med.kochi-u.ac.jp OBJECTIVE: To investigate the macrophage response in endometriosis by determining the expression and localization of human leukocyte antigen (HLA)-ABC and HLA-DR by the peritoneal fluid (PF) macrophages and PF concentrations of interferon (IFN)-gamma that regulate HLA expression. DESIGN: Case-control study. SETTING: University hospital. PATIENT(S): 64 Japanese endometriosis patients, and 65 women with other laparoscopic diagnoses. INTERVENTION(S): Venipuncture and laparoscopic peritoneal fluid collection. MAIN OUTCOME MEASURE(S): Expression and localization of HLA-ABC and HLA-DR in PF macrophages were determined by flow cytometry and confocal microscopy. The concentration of IFN-gamma in PF was determined by enzyme-linked immunosorbent assay. RESULT(S): In women with endometriosis, expression of HLA-ABC and HLA-DR by PF macrophages, and the IFN-gamma concentrations in PF were statistically significantly lower than in controls. Women with endometriosis showed a statistically significant positive correlation between HLA expression and IFN-gamma concentration. By confocal microscopy, HLA-ABC was distributed homogenously on the macrophage surface whereas HLA-DR expression on these cells corresponded to the lipid raft. CONCLUSION(S): In women with endometriosis, low HLA expression and particularly reduced HLA-DR in the lipid raft may be influenced by low IFN-gamma and may compromise antigen presentation, limiting the immune response to peritoneal cavity antigens such as implanted or metaplastic endometrial tissue. Mesh Terms: Adult| Antigen Presentation| Ascitic Fluid/*immunology| Case-Control Studies| Down-Regulation| Endometriosis/*immunology| Enzyme-Linked Immunosorbent Assay| Female| Flow Cytometry| HLA-DR Antigens/*analysis| Histocompatibility Antigens Class I/* |
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