Results
| PMID | 17693327 |
| Gene Name | CCR8 |
| Condition | Endometriosis |
| Association |
Associated |
| Sex | Female |
| Other associated phenotypes |
Endometriosis |
|
Fertil Steril. 2007 Aug;88(2):317-25. doi: 10.1016/j.fertnstert.2006.11.129. Shi, Ying-Li| Luo, Xue-Zhen| Zhu, Xiao-Yong| Li, Da-Jin Laboratory for Reproductive Immunology, Hospital and Institute of Obstetrics and Gynecology, Fudan University Shanghai Medical College, Shanghai, China. OBJECTIVE: To explore the effects of the combined E(2) with the environmental pollutant 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on CCR8-I-309 expression by the endometriotic lesion-associated cells in the pathogenesis of endometriosis. DESIGN: Prospective laboratory study. SETTING: University hospital. PATIENT(S): Chinese women with endometriosis. INTERVENTION(S): The endometriotic tissue and matched eutopic endometrium were collected. Endometrial stromal cells (ESCs), HPMC, and U937 cells were treated with 17beta-E(2) or TCDD. The ESCs were stimulated with I-309. MAIN OUTCOME MEASURE(S): The expression of CCR8 in tissues was analyzed by reverse transcription-polymerase chain reaction and immunohistochemistry. The effect of I-309 on integrin beta1 and alphavbeta3 expression intensity was analyzed by flow cytometry, and the chemotactic activity of I-309 on the ESC was explored by chemotactic assay. Concentration of I-309 in the culture supernatant was quantified by enzyme-linked immunosorbent assay. RESULT(S): CCR8 was overexpressed in the endometriotic tissue. I-309 promoted the expression of integrin beta1. Estradiol and TCDD up-regulated CCR8 expression by ESCs. Estradiol magnified the stimulatory effect of TCDD on I-309 secretion by U937. The interaction of HPMC and U937 cells promoted I-309 secretion. CONCLUSION(S): These findings imply that the combination of 17beta-E(2) with the environmental pollutant TCDD is involved in the pathogenesis of endometriosis via up-regulating the chemokine CCR8-I-309. Mesh Terms: Cells, Cultured| Chemokine CCL1| Chemokines, CC/*genetics/metabolism| Drug Combinations| Endometriosis/*chemically induced/genetics/metabolism| Endometrium/metabolism| Environmental Pollutants/administration & dosage/toxicity| Estradiol/administrat |
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