Results
| PMID | 17952761 |
| Gene Name | MMP24 |
| Condition | Endometriosis |
| Association |
Associated |
| Sex | Female |
| Associated genes | MT5-MMP |
| Other associated phenotypes |
Endometriosis |
|
Gynecol Endocrinol. 2007 Oct;23(10):567-73. Gaetje, Regine| Holtrich, Uwe| Engels, Knut| Kourtis, Katherina| Cikrit, Eva| Kissler, Stefan| Rody, Achim| Karn, Thomas| Kaufmann, Manfred Department of Obstetrics and Gynecology, Johann Wolfgang Goethe-University, Frankfurt, Germany. gaetje@em.uni-frankfurt.de BACKGROUND: The metalloproteinases (MMPs) are a family of proteolytic enzymes involved in tissue remodeling and cell migration. Endometrial tissue remodeling proceeds during the menstrual cycle and requires a temporary and spatially balanced expression of several different MMPs. Various members of the MMPs also seem to play an important role in the invasion process of endometriosis; however, so far only a limited number of studies have focused on membrane-associated MMPs. METHODS: The present study investigated the expression of membrane-type 5 metalloproteinase (MT5-MMP) in the human endometrium and endometriotic lesions by microarray hybridization, real-time polymerase chain reaction (PCR) and immunofluorescence. RESULTS: Both the gene chip expression analyses as well as PCR indicated expression of MT5-MMP in normal human endometrium and strongly elevated transcript levels in most peritoneal endometriosis lesions analyzed. Moreover we detected enhanced MT5-MMP expression in the eutopic endometrium from patients suffering from endometriosis, further supporting a role of MT5-MMP in the formation of endometriosis. Immunohistochemical analysis was used to determine the intracellular localization and tissue distribution of MT5-MMP. While the MT5-MMP antigen expression could be clearly attributed to the membrane of epithelial cells, a highly complex differential immunohistochemical staining of MT5-MMP in the various compartments of endometrial tissue was observed. The strongest staining was seen in luminal epithelial cells, whereas endometrial glands frequently showed partial expression of MT5-MMP. CONCLUSION: Our microarray analysis and real-time PCR of MT5-MMP transcripts may point to an elevated tissue remodeling and cell migration in endometrium from endometriosis patients as implied by the function of related MMPs. Mesh Terms: Endometriosis/*metabolism| Endometrium/*metabolism| Endothelial Cells/metabolism| Female| Gene Expression Profiling| Humans| Matrix Metalloproteinases, Membrane-Associated/genetics/*metabolism| *Oligonucleotide Array Sequence Analysis|DA 2008/01/04 |
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