Results
| PMID | 17953945 |
| Gene Name | IL6 |
| Condition | Endometriosis |
| Association |
Associated |
| Population size | 12 |
| Population details | 12 (6 ectopic endometriosis, 6 eutopic endometriosis) |
| Sex | Female |
| Associated genes | IL-6 |
| Other associated phenotypes |
Ectopic endometriosis |
|
Fertil Steril. 2008 May;89(5 Suppl):1578-84. Epub 2007 Oct 22. Salmassi, Ali| Acil, Yahya| Schmutzler, Andreas Gerd| Koch, Kerstin| Jonat, Walter| Mettler, Liselotte Department of Obstetrics and Gynecology, University Hospitals Schleswig-Holstein, Campus Kiel, Kiel, Germany. endo-office@email.uni-kiel.des OBJECTIVE: To evaluate the concentration and distribution pattern of interleukin-6-producing cells in ectopic and eutopic endometrium. DESIGN: Collection of both tissues at the proliferative cycle phase. SETTING: A university endocrinology and laparoscopy program. PATIENT(S): Twelve samples from six women were obtained at operative laparoscopy in the proliferative phase of each woman's cycle. INTERVENTION(S): Paired sampling of ectopic (n = 6) and eutopic endometrium (n = 6). MAIN OUTCOME MEASURE(S): Concentration of IL-6 messenger RNA (mRNA) was measured by real-time RT-PCR, and its distribution was determined by in situ RT-PCR. RESULT(S): In vitro RT-PCR analysis showed distinct bands at 234 bp and 115 bp for IL-6 mRNA in eutopic and ectopic endometrium and in placenta as positive control. Real-time RT-PCR indicated significantly higher expression of IL-6 in tissue with endometriosis than in tissue without. In all 12 samples subjected to in situ RT-PCR, positive signals of IL-6 mRNA were observed in cytoplasm of epithelial and stroma cells of ectopic and eutopic endometrium tissue. CONCLUSION(S): The significant IL-6 mRNA increase in endometriosis compared with in eutopic endometrium may play a role in the development of endometriosis and may provide new directions for endometriosis research. Mesh Terms: Adult| Endometriosis/*genetics/metabolism/pathology| Endometrium/*metabolism| Female| Gene Expression Regulation| Humans| Interleukin-6/*genetics/metabolism| RNA, Messenger/*metabolism| Reverse Transcriptase Polymerase Chain Reaction| Tissue Dist |
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