Results
| PMID | 18684444 |
| Gene Name | GHRH |
| Condition | Endometriosis |
| Association |
Associated |
| Sex | Female |
| Associated genes | GHRH, SV1 |
| Other associated phenotypes |
Endometriosis |
|
Fertil Steril. 2009 Jul;92(1):47-53. doi: 10.1016/j.fertnstert.2008.04.048. Epub Fu, Li| Osuga, Yutaka| Yano, Tetsu| Takemura, Yuri| Morimoto, Chieko| Hirota, Yasushi| Schally, Andrew V| Taketani, Yuji Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, Japan. OBJECTIVE: To determine possible involvement of splice variant 1 (SV1), a variant of the pituitary growth hormone-releasing hormone (GHRH) receptor, in the development of endometriosis. DESIGN: Comparative and laboratory study. SETTING: University teaching hospital reproductive endocrinology and infertility practice. PATIENT(S): Eutopic and ectopic endometrial tissues, and peritoneal bone marrow-derived cells were collected from women with or without endometriosis. Normal ovarian tissues were collected from women without endometriosis. INTERVENTION(S): Ectopic endometrial stromal cells (ESC) were isolated and cultured with or without GHRH. MAIN OUTCOME MEASURE(S): Gene expression of GHRH and SV1 in the sample tissues was determined by reverse transcriptase (RT) nested polymerase chain reaction (PCR). Cyclic adenosine monophosphate (cAMP) production and 5-bromo-2'-deoxyuridine (BrdU) incorporation in ESC were measured using specific assay systems. RESULT(S): We detected SV1 messenger RNA (mRNA) in 17 out of 27 (63%) ectopic endometrial tissues, which was statistically significantly higher than that detected in eutopic endometrial tissues (2 out of 47, 4%) and normal ovarian tissues (0 out of 14). A relatively low rate of GHRH mRNA was detected in ectopic endometrial tissues (6 out of 27, 24%) and in eutopic endometrial tissues (12 out of 47, 26%). In contrast, relatively high rates were detected in normal ovarian tissues (14 out of 14, 100%) and peritoneal bone marrow-derived cells (13 out of 16, 81%). We found that GHRH stimulated the production of cAMP and the incorporation of BrdU in SV1-expressing ESC. CONCLUSION(S): GHRH and SV1 may play a role in promoting the development of endometriosis. Mesh Terms: Adult| *Alternative Splicing| Antigens, CD45| Bone Marrow Cells/pathology| DNA Primers| Endometriosis/*genetics/surgery| Female| Gene Expression Regulation| *Genetic Variation| Growth Hormone-Releasing Hormone/genetics| Humans| Laparoscopy| Me |
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