Results
| PMID | 18955785 |
| Gene Name | ANG |
| Condition | Endometriosis |
| Population size | 61 |
| Population details | 61 (32 women with endometriosis and 29 control women) |
| Sex | Female |
| Other associated phenotypes |
Endometriosis, Implantation failure |
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J Korean Med Sci. 2008 Oct;23(5):802-7. doi: 10.3346/jkms.2008.23.5.802. Kim, Sung Hoon| Choi, Young Min| Chae, Heen Dong| Kim, Chung Hoon| Kang, Byung Moon Department of Obstetrics and Gynecology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea. Angiogenin, a potent inducer of angiogenesis, is expressed in human endometrium. This study was performed to compare the expression of angiogenin mRNA level in the eutopic endometrium from women with and without endometriosis. Thirty-two women with advanced stage endometriosis and 29 control women were recruited. Following isolation of total RNA from endometrial tissue and reverse transcription, cDNA samples were amplified by real time polymerase chain reaction to quantify the expression of angiogenin genes. In selected patients, immunohistochemical staining was utilized to localize the area of angiogenin expression. Angiogenin mRNA level was significantly lower in the endometriosis group than in the control group during the secretory phase, especially the mid-secretory phase, and the decline was observed mainly in the women who presented with infertility. Within the endometriosis group, angiogenin mRNA levels did not differ between the proliferative and secretory phases, but, in the control group, the level in the secretory phase was higher than that during the proliferative phase. Immunohistochemistry showed that the glandular epithelial cell layer was decorated positively in both groups. These findings suggest that the relative deficiency of angiogenin expression in the secretory endometrium could impair implantation in women with advanced stage endometriosis. Mesh Terms: Adult| Endometriosis/*metabolism/pathology| Endometrium/*metabolism| Female| Fertility| *Gene Expression Regulation| Humans| Immunohistochemistry/methods| Menstrual Cycle| Models, Biological| RNA, Messenger/metabolism| Reverse Transcriptase Pol |
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