Results
| PMID | 20617440 |
| Gene Name | CCL5 |
| Condition | Endometriosis |
| Association |
Associated |
| Population size | 20 |
| Population details | 20 (12 with endometriosis(ePF), 8 controls without endometriosis (cPF)) |
| Sex | Female |
| Associated genes | RANTES |
| Other associated phenotypes |
Endometriosis |
|
Arch Gynecol Obstet. 2011 Jun;283(6):1333-41. doi: 10.1007/s00404-010-1583-1. Na, Yong-Jin| Lee, Dong-Hyung| Kim, Seung-Chul| Joo, Jong-Kil| Wang, Ji-Won| Jin, Jun-O| Kwak, Jong-Young| Lee, Kyu-Sup Department of Obstetrics and Gynecology, Medical Research Institute, Pusan National University, Busan 602-739, Korea. PURPOSE: Chemokines have been implicated in the pathological process of endometriosis. We compared the effects of peritoneal fluid obtained from patients with endometriosis (ePF) and controls without endometriosis (cPF) on the release of monocyte-specific CC chemokines such as monocyte chemotactic protein-1 (MCP-1), regulated upon activation normal T cell expressed and secreted (RANTES), and macrophage inflammatory protein-1alpha (MIP-1alpha) by neutrophils, monocytes, and T cells. Moreover, we evaluated the correlation between the levels of chemokines in ePF and their release by these cells. METHODS: Cells were obtained from healthy young volunteers and cultured with ePF (n = 12) or cPF (n = 8). The chemokine levels in the ePF and the supernatants of cultured cells with ePF were then measured by ELISA. RESULTS: There was a positive correlation between the levels of MCP-1 and MIP-1alpha in ePF. The addition of ePF to the cell cultures failed to increase the release of MCP-1, RANTES, and MIP-1alpha when compared to cPF, but the levels of RANTES in ePF were positively correlated with the release of RANTES by ePF-treated monocytes and T cells. Moreover, there was a positive correlation between the levels of RANTES and MIP-1alpha released by neutrophils and between the levels of MCP-1 and MIP-1alpha released by T cells. Finally, the levels of RANTES released by monocyte-derived macrophages and monocytes cultured with ePF were positively correlated. CONCLUSIONS: These findings suggest that monocytes, neutrophils, and T cells release differential levels of MCP-1, RANTES, and MIP-1alpha in response to stimulation with ePF. Mesh Terms: Ascitic Fluid/*immunology| CD4-Positive T-Lymphocytes/immunology| CD8-Positive T-Lymphocytes/immunology| Cells, Cultured| Chemokine CCL2/metabolism| Chemokine CCL3/metabolism| Chemokine CCL5/metabolism| Chemokines, CC/*metabolism| Endometriosis/*i |
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