Results
| PMID | 21601196 |
| Gene Name | PTGS2 |
| Condition | Endometriosis |
| Association |
Associated |
| PMID | 21601196 |
| Gene Name | COX2 |
| Condition | Endometriosis |
| Association |
Associated |
| Sex | Female |
| Associated genes | IL-17F, IL-8, COX2 |
| Other associated phenotypes |
Endometriosis |
|
Fertil Steril. 2011 Jul;96(1):113-7. doi: 10.1016/j.fertnstert.2011.04.060. Epub Hirata, Tetsuya| Osuga, Yutaka| Takamura, Masashi| Saito, Ako| Hasegawa, Akiko| Koga, Kaori| Yoshino, Osamu| Hirota, Yasushi| Harada, Miyuki| Taketani, Yuji Department of Obstetrics and Gynecology, University of Tokyo, Tokyo, Japan. OBJECTIVE: To examine the effects of interleukin (IL)-17F on the secretion of IL-8 and the gene expression of cyclooxygenase 2 (COX2) in endometriotic stromal cells. DESIGN: In vitro experimental study using human samples. SETTING: University hospital. PATIENT(S): Endometriotic tissues were obtained from women with ovarian endometriomas undergoing laparoscopic surgery. INTERVENTION(S): Endometriotic stromal cells (ESCs) were cultured with IL-17F. MAIN OUTCOME MEASURE(S): Concentrations of IL-8 were measured by a specific ELISA, and messenger RNA levels of IL-8 and COX2 were measured by real-time reverse transcription-polymerase chain reaction (PCR). RESULT(S): IL-17F increased the secretion of IL-8 from ESCs, and the effect was inhibited by antibodies for IL-17 receptor A and IL-17 receptor C. Tumor necrosis factor alpha (TNF-alpha) synergistically enhanced IL-17F-induced increase in IL-8 secretion from ESCs. The IL-17F increased the gene expression of IL-8 and COX2 in ESCs. CONCLUSION(S): These findings suggest that IL-17F may stimulate the development of endometriosis by up-regulation of IL-8 and COX2. Mesh Terms: Cells, Cultured| Cyclooxygenase 2/*biosynthesis| Endometriosis/enzymology/*metabolism| Endometrium/enzymology/secretion| Female| *Gene Expression Regulation, Enzymologic| Humans| Interleukin-17/*physiology| Interleukin-8/*secretion|DA 2011/08/30 0 |
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