Results
| PMID | 22095791 |
| Gene Name | YBX1 |
| Condition | Endometriosis |
| Association |
Associated |
| Population size | 211 |
| Population details | 211 (120 women with endometriosis, 91 women without endometriosis) |
| Sex | Female |
| Associated genes | YB-1 |
| Other associated phenotypes |
Endometriosis |
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Hum Reprod. 2012 Jan;27(1):173-82. doi: 10.1093/humrep/der368. Epub 2011 Nov 16. Silveira, C G T| Krampe, J| Ruhland, B| Diedrich, K| Hornung, D| Agic, A Department of Obstetrics and Gynecology, University of Schleswig-Holstein, Campus Luebeck, Ratzeburgerallee 160, 23538 Luebeck, Germany. BACKGROUND: The Y-box-binding protein (YB-1) is described as a potential oncogene highly expressed in tumors and associated with increased cell survival, proliferation, migration and anti-apoptotic signaling. The aim of our study was to examine the expression and role of YB-1 in human endometriosis (Eo) and its association with cell survival, proliferation and invasion. METHODS: We analyzed the gene and protein expression levels of YB-1 by quantitative real-time RT-PCR and immunoassays, respectively, in peritoneal macrophages, ovarian endometrioma and eutopic endometrial tissues/cells derived from women with (n= 120) and without (n= 91) Eo. We also evaluated the functional consequences of YB-1 knockdown in the Z12 Eo cell line by measuring cell proliferation [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromid cell proliferation assay], invasion (Matrigel invasion assay) and spontaneous and tumour necrosis factor (TNFalpha)-induced RANTES (regulated upon activation, normal T-cell expressed and secreted chemokine) expression and apoptosis (ELISA-based assay). RESULTS: YB-1 gene and protein expression was statistically significantly higher in ovarian lesions, eutopic endometrium and peritoneal macrophages of patients with Eo in comparison with the control group. Interestingly, the strongest YB-1 expression was observed in the epithelial compartment of endometrial tissues. In the Z12 cell line, YB-1 knockdown resulted in significant cell growth inhibitory effects including reduced cell proliferation and increased rates of spontaneous and TNFalpha-induced apoptosis. Significantly, higher RANTES expression and decreased cell invasion in vitro were also associated with YB-1 inactivation. CONCLUSION: High YB-1 expression could have an impact on the development and progression of Eo. This study suggests the role of YB-1 as a potential therapeutic target for Eo patients. Mesh Terms: Adult| Apoptosis| Cell Proliferation| Cell Survival| Chemokine CCL5/metabolism| Collagen/chemistry| Drug Combinations| Endometriosis/*metabolism| Endometrium/*metabolism| Female| *Gene Expression Regulation| Humans| Inflammation| Laminin/chem |
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