Results
| PMID | 22356900 |
| Gene Name | GATA3 |
| Condition | Endometriosis |
| Association |
Associated |
| Population size | 40 |
| Population details | 40 (20 women with laparoscopically confirmed endometriosis, 20 women without endometriosis) |
| Sex | Female |
| Associated genes | T-bet, GATA-3 |
| Other associated phenotypes |
Endometriosis |
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Acta Histochem. 2012 Dec;114(8):779-84. doi: 10.1016/j.acthis.2012.01.005. Epub Chen, Peng| Zhang, Zhifang| Chen, Qi| Ren, Fang| Li, Tong| Zhang, Chiyuan| Wang, Danbo Department of Obstetrics and Gynecology, Shengjing Hospital Affiliated to China Medical University, Shenyang, People's Republic of China. The objective of the present study was to evaluate the expression of T-box expressed in T cells (T-bet) and GATA binding protein 3 (GATA-3) in the eutopic endometrium from women with endometriosis. Endometrial tissues were collected from 20 women with laparoscopically confirmed endometriosis and 20 women without endometriosis. T-bet and GATA-3 expression was measured by quantitative real-time PCR (qPCR), immunohistochemistry and Western blot analysis. Eutopic endometrial tissues from patients with endometriosis expressed lower levels of T-bet mRNA and high levels of GATA-3 mRNA, leading to a significant lower T-bet/GATA-3 mRNA ratio (P<0.05). Western blot analysis showed that the T-bet/GATA-3 protein ratio in endometriosis group was also statistically lower than that in the control group (P<0.05). These results suggested that T-bet and GATA-3 may act as cytokine regulatory genes, and the Th2-specific transcription factor, GATA-3, probably plays an essential role in the immune response and the development of endometriosis. Mesh Terms: Adult| Blotting, Western| Cytokines/*genetics| Endometriosis/*genetics/pathology| Endometrium/*metabolism/pathology| Female| GATA3 Transcription Factor/*analysis/*genetics| Humans| Immunohistochemistry| RNA, Messenger/analysis/genetics| Real-Tim |
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