Results
| PMID | 22608095 |
| Gene Name | PTGS2 |
| Condition | Endometriosis |
| Association |
Associated |
| Population size | 80 |
| Population details | 80 (60 women with endometriosis (endometriosis group) 20 women without endometriosis (control group)) |
| Sex | Female |
| Other associated phenotypes |
Endomertiosis |
|
Eur J Med Res. 2012 May 18;17:12. doi: 10.1186/2047-783X-17-12. Wang, DanBo| Chen, Qi| Zhang, Chiyuan| Ren, Fang| Li, Tong Department of Obstetrics & Gynecology, Shengjing Hospital Affiliated to China Medical University, Shenyang, People's Republic of China. wangdb@sj-hospital.org BACKGROUND: Accumulated evidence reveals that cyclooxygenase-2 (COX-2) was overexpressed in eutopic endometrium of endometriosis, which may play a critical role in the pathogenesis of endometriosis. However, few studies have been performed to explore the molecular mechanisms underlying the abnormal high expression of COX-2 in endometriosis. Considering the fact that a number of recent studies have shown DNA methylation affecting some genes in endometriosis, the present study was therefore aimed to determine whether the observed high expression COX-2 in endometriosis is caused by the hypomethylation of CpG island within the promoter of this gene. METHODS: The endometrial tissues were collected from 60 women with endometriosis (endometriosis group) and 20 women without endometriosis (control group). The methylation status of COX-2 was examined by methylation specific PCR. Quantitative real-time RT-PCR was performed to measure COX-2 mRNA level in endometrial tissues. RESULTS: The frequency of promoter hypermethylation of COX-2 was lower in eutopic endometrium of the endometriosis group (41.7%) than that in the control group (75.0%), P < 0.05. COX-2 mRNA level in the eutopic endometrium of the endometriosis group was 2.61-fold higher than that in the control group (P < 0.01). COX-2 mRNA level in unmethylated endometrium of the endometriosis group or the control group was 2.39-fold and 2.66-fold, respectively, higher than that in the methylated endometrium of the same group (P < 0.01). CONCLUSIONS: The hypomethylation within the promoter of COX-2 may be responsible for the elevated gene expression in eutopic endometrium of endometriosis. Mesh Terms: Adult| CCAAT-Enhancer-Binding Protein-delta/genetics/metabolism| Case-Control Studies| CpG Islands| Cyclooxygenase 2/genetics/*metabolism| *DNA Methylation| Endometriosis/*genetics/pathology| Endometrium/metabolism/*pathology| Epigenesis, Genetic| |
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