Results
| PMID | 23269356 |
| Gene Name | DEFB4A |
| Condition | Endometriosis |
| Association |
Associated |
| Population size | 50 |
| Population details | 50 women including EMS patients undergoing laparoscopic ovarian cystectomy, non-EMS patients undergoing hysterectomy for uterine fibroids |
| Sex | Female |
| Associated genes | hBD-2, TNFA, IL-1b |
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Arch Gynecol Obstet. 2013 Jun;287(6):1151-7. doi: 10.1007/s00404-012-2686-7. Epub Chen, Shu-Qin| Li, Jin-Bo| Jiang, Hong-Ye| Yuan, Li| Niu, Gang| Yao, Shu-Zhong Department of Gynecology and Obstetrics, The First Affiliated Hospital, Sun Yat-sen University, No. 58, Zhongshan 2th Road, 510080 Guangzhou, China. chenshuqin1021@yahoo.com.cn OBJECTIVE: To investigate the expression of human beta-defensin-2 (hBD-2) in the endometrium of patients with endometriosis (EMS) and explore the potential role of hBD-2 in the pathogenesis of EMS. DESIGN: Prospective controlled study. SAMPLE: 50 women including EMS patients undergoing laparoscopic ovarian cystectomy and non-EMS patients undergoing hysterectomy for uterine fibroids. SETTING: Large university teaching hospital. METHODS: Patients were divided into EMS and non-EMS groups. The gene expressions of hBD-2, interleukin (IL)-1beta and tumor necrosis factor (TNF)-alpha in the endometrial tissues of each group were detected with real-time quantitative polymerase chain reaction (PCR), and hBD-2 protein expression with immunohistochemical method. RESULTS: The gene expression levels of hBD-2, TNF-alpha, and IL-1beta as well as the positive expression rate of hBD-2 protein in the ectopic endometrium of EMS patients were significantly higher than those in the eutopic endometrium of EMS and non-EMS patients (all P < 0.05). Correlation analysis showed that the gene expression levels of hBD-2 in the ectopic and eutopic endometrium of EMS patients were positively correlated with the gene expression levels of IL-1beta and TNF-alpha (P < 0.01). CONCLUSION: High levels of hBD-2 gene and protein expressions in the ectopic endometrium of EMS patients may be an important contributor in the pathogenesis of EMS. TNF-alpha and IL-1beta may promote the upregulation of hBD-2 expression. Mesh Terms: Adult| Endometriosis/*metabolism| Endometrium/*chemistry| Female| Gene Expression| Humans| Immunohistochemistry| Interleukin-1beta/genetics| Prospective Studies| RNA, Messenger/analysis| Real-Time Polymerase Chain Reaction| Tumor Necrosis Facto |
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