Results
| PMID | 23466190 |
| Gene Name | CTSB |
| Condition | Endometriosis |
| Association |
Associated |
| Population size | 55 |
| Population details | 55 (31 patients with endometriosis (stages I-IV), 24 controls) |
| Sex | Female |
| Associated genes | cathepsins B, cathepsins D, cathepsins G |
| Other associated phenotypes |
Endometriosis |
|
Eur J Obstet Gynecol Reprod Biol. 2013 Jul;169(1):80-3. doi: Laudanski, Piotr| Gorodkiewicz, Ewa| Ramotowska, Barbara| Charkiewicz, Radoslaw| Kuzmicki, Mariusz| Szamatowicz, Jacek Department of Perinatology, Medical University of Bialystok, ul. Marii Sklodowskiej-Curie 24a, Bialystok, Poland. plauda@umb.edu.pl OBJECTIVE: To determine the concentrations of cathepsins B, D and G in proliferative eutopic endometrium of patients with and without endometriosis, by use of the surface plasmon resonance imaging (SPRI) technique. STUDY DESIGN: A total of 55 patients were recruited in the study: 31 patients with endometriosis (stages I-IV) and 24 controls. Endometrial samples were obtained in the first phase of the menstrual cycle from regularly menstruating premenopausal women, prior to laparoscopy, by the use of aspiration biopsy. Endometriosis was appropriately classified according to the Revised American Fertility Society classification and confirmed by histopathology in every case. The SPRI technique was used to determine the concentration of cathepsins B, D and G. To compare the two groups for quantitative data, Mann-Whitney-Wilcoxon's test was used due to the non-normal distribution of the tested variables and normality of distribution was assessed using Shapiro-Wilk W test. RESULTS: The concentration of the three examined cathepsins was higher in the proliferative eutopic endometrium of patients with endometriosis, especially in advanced stages, e.g. III and IV, when compared to healthy individuals. Corresponding median values were, for cathepsin B: [7.93 pmol/mg (min-max 2.82-15.71) vs 1.2 pmol/mg (min-max 0.7-15.49) p=0.0014], for cathepsin D: [1.86 pmol/mg (min-max 0.51-5.4) vs 1.03 pmol/mg (min-max 0.4-2.72) p=0.00041] and for cathepsin G: [0.6 pmol/mg (min-max 0.33-2.51) vs 0.3 pmol/mg (min-max 0.16-1.29) p=0.00051]. CONCLUSIONS: Increased concentrations of cathepsins B, D and G in the proliferative eutopic endometrium may play a role in the implantation of endometrial tissue outside the uterine cavity. Mesh Terms: Adult| Cathepsin B/*metabolism| Cathepsin D/*metabolism| Cathepsin G/*metabolism| Endometriosis/*metabolism/pathology| Endometrium/*metabolism/pathology| Female| Humans| Surface Plasmon Resonance|DA 2014/01/22 06:00 |
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