Results
| PMID | 24963168 |
| Gene Name | HOXA10 |
| Condition | Endometriosis |
| Association |
Associated |
| Population size | 30 |
| Population details | 30 (18 women admitted for surgery for endometriosis-related pain (cases), 12 women admitted for surgery because of non-endometriotic disease (control)) |
| Sex | Female |
| Other associated phenotypes |
Endometriosis |
|
Hum Reprod. 2014 Sep;29(9):1906-11. doi: 10.1093/humrep/deu161. Epub 2014 Jun 24. Andersson, K L| Bussani, C| Fambrini, M| Polverino, V| Taddei, G L| Gemzell-Danielsson, K| Scarselli, G ISPO Cancer Prevention and Research Institute, Via Cosimo Il Vecchio 2, Florence 50139, Italy Department of Women's and Children's Health, Division of Obstetrics and Gynaecology, Karolinska Institutet/Karolinska University Hospital, Solna 17176, Swed STUDY QUESTION: Does the methylation status of the promoter region of the HOXA10 gene differ in eutopic and ectopic endometrium? SUMMARY ANSWER: The eutopic endometrium in women with endometriosis is significantly more methylated when compared with controls. WHAT IS KNOWN ALREADY: Expression of the HOXA10 gene, which is important for successful implantation, is reduced in women affected by endometriosis. STUDY DESIGN, SIZE AND DURATION: A pilot study was carried out including 18 women admitted for surgery for endometriosis-related pain (cases) and 12 women admitted for surgery because of non-endometriotic disease (control). Sample collection and analysis were performed between November 2010 and July 2013. PARTICIPANTS/MATERIALS, SETTING, METHODS: Endometrial tissue (eutopic and ectopic) underwent sodium bisulfite DNA modification, PCR amplification of two regions of the HOXA10 promoter and pyrosequencing analysis. MAIN RESULTS AND THE ROLE OF CHANCE: The eutopic endometrium of women with endometriosis was significantly more methylated compared with endometrium from the control group (sequence 1: 8.68% in cases and 6.25% in the control group: P = 0.037, sequence 2: 11.89% in cases and 9.25% in the control group: P = 0.032). The eutopic endometrium was significantly more methylated than the ectopic tissue in patients with endometriosis (mean difference -3.6 sequence 1: P = 0.001 and -6.0 sequence 2: P = 0.0001). LIMITATIONS, REASONS FOR CAUTION: The study had a limited sample size and the fertility status of the majority of patients in our study was unknown. WIDER IMPLICATIONS OF THE FINDINGS: Our data regarding methylation state of the ectopic tissues contribute to a better etiopathologic understanding of endometriosis. STUDY FUNDING/COMPETING INTERESTS: No external funding was either sought or obtained for this study. The authors have no conflicts of interests to declare. Mesh Terms: Adult| *DNA Methylation| Endometriosis/*genetics| Endometrium/metabolism/*pathology| Female| Homeodomain Proteins/*genetics/metabolism| Humans| Pilot Projects|DA 2015/04/24 06:00 |
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