Results
| PMID | 25977120 |
| Gene Name | IL33 |
| Condition | Endometriosis |
| Association |
Associated |
| Population size | 50 |
| Population details | 50 (30 women with laparoscopic and histopathological confirmed endometriosis, 20 control women without pelvic pathology) |
| Sex | Female |
| Associated genes | ST2, IL-33 |
| Other associated phenotypes |
Endometriosis |
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Immunol Lett. 2015 Jul;166(1):1-5. doi: 10.1016/j.imlet.2015.05.002. Epub 2015 Mbarik, Maroua| Kaabachi, Wajih| Henidi, Balkis| Sassi, Faycal Haj| Hamzaoui, Kamel Tunis El Manar University, Medicine Faculty of Tunis, Department of Immunology and Histology, Tunisia. Electronic address: marwambarik@gmail.com.| Tunis El Manar University, Medicine Faculty of Tunis, Department of Immunology and Histology, Tunisia; A. Interleukin-33 is an IL-1 family cytokine which signals via its T1/ST2 receptor, and acts as a key regulator of inflammation. This study aims to measure the expression of soluble ST2 (sST2) and IL-33 in endometriosis. We investigated thirty women with laparoscopic and histopathological confirmed endometriosis and 20 control women without pelvic pathology. Peripheral blood mononuclear cells and peritoneal fluid (PF) were assessed for sST2 and IL-33 levels that are measured by sandwich enzyme-linked immunosorbent assay. Peritoneal fluid IL-33 mRNA expression was quantified by real-time reverse transcription polymerase chain reaction assays. We found that IL-33 levels in PF and in serum were significantly higher in patients with endometriosis compared to women without endometriosis (P < 0.05). IL-33 increased levels were significantly more important in PF [10.45 +/- 14.33 ng/mL] than in serum [2.68 +/- 1.54 ng/mL] from endometriosis patients. Higher levels of IL-33 mRNA expression were detected in PF from patients with endometriosis. Soluble ST2 levels in PF were significantly different between patients [2.96 +/- 0.98 ng/mL; P < 0.0001] and controls [0.88 +/- 0.076 ng/mL]. Serum sST2 levels were similarly expressed in endometriosis patients and in controls (P > 0.05). Significant correlation was observed between IL-33 and sST2 levels in PF. In conclusion, IL-33 and sST2 values observed in PF were found to correlate with endometriosis severity. Elevated and correlated PF IL-33 and sST2 levels from patients with endometriosis suggested a potential role as surrogate markers of disease activity. Mesh Terms: Ascitic Fluid/*metabolism| Biomarkers/blood/metabolism| Endometriosis/diagnosis/genetics/*pathology| Enzyme-Linked Immunosorbent Assay| Female| Humans| Inflammation| Interleukin-1 Receptor-Like 1 Protein| Interleukin-33/blood/genetics/*metabolism| |
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