Results
| PMID | 27179784 |
| Gene Name | MIR125B1 |
| Condition | Endometriosis |
| Association |
Associated |
| Population size | 48 |
| Population details | 48 (24 patients with endometriosis, 24 patients without endometriosis) |
| Sex | Female |
| Associated genes | miR-3613-5p, miR-6755-3p, miR-125b-5p, miR-150-5p, miR-342-3p, miR-143-3p, miR-145-5p, miR-500a-3p, miR-451a, miR-18a-5p |
| Other associated phenotypes |
Endometriosis |
|
Fertil Steril. 2016 Aug;106(2):402-9. doi: 10.1016/j.fertnstert.2016.04.013. Epub Cosar, Emine| Mamillapalli, Ramanaiah| Ersoy, Gulcin Sahin| Cho, SihYun| Seifer, Benjamin| Taylor, Hugh S Department of Obstetrics, Gynecology, and Reproductive Sciences, Yale School of Medicine, New Haven, Connecticut.| Department of Obstetrics, Gynecology, and Reproductive Sciences, Yale School of Medicine, New Haven, Connecticut. Electronic address: ra OBJECTIVE: To investigate serum microRNAs (miRNAs) in women with endometriosis. DESIGN: Case-control study. SETTING: University hospital. PATIENT(S): Women with (n = 24) and without (n = 24) endometriosis. INTERVENTION(S): Serum samples were obtained from surgically diagnosed subjects. MAIN OUTCOME MEASURE(S): miRNA from women with without endometriosis were used for microarray profiling and confirmed by means of quantitative real-time polymerase chain reaction (qRT-PCR). Receiver operating characteristic (ROC) analysis was performed on differentially expressed miRNAs. RESULT(S): miR-3613-5p, miR-6755-3p were down-regulated and miR-125b-5p, miR-150-5p, miR-342-3p, miR-143-3p, miR-145-5p, miR-500a-3p, miR-451a, miR-18a-5p were up-regulated more than 10-fold in the microarray. These results were confirmed with the use of qRT-PCR. Among the differentially expressed miRNAs, miR-125b-5p expression levels had the highest area under the ROC curve (AUC). The maximum AUC score of 1.000 was achieved when combining miR-125b-5p, miR-451a, and miR-3613-5p with the use of a logistic regression model. CONCLUSION(S): We identified several miRNAs in serum that distinguished subjects with endometriosis from those without. miR-125b-5p had the greatest potential as a single diagnostic biomarker. A combination of that miRNA with miR-451a and miR-3613-5p further improved diagnostic performance. Mesh Terms: Adult| Area Under Curve| Case-Control Studies| Connecticut| Endometriosis/*blood/diagnosis/genetics| Female| Gene Expression Profiling/*methods| Genetic Markers| Genetic Predisposition to Disease| Hospitals, University| Humans| Logistic Models| |
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