Results
| PMID | 27793384 |
| Gene Name | MRC1 |
| Condition | Endometriosis |
| Association |
Associated |
| Population size | 63 |
| Population details | 63 (35 patients with endometriosis, 28 control women) |
| Sex | Female |
| Other associated phenotypes |
Endometriosis |
|
Fertil Steril. 2017 Jan;107(1):167-173.e2. doi: 10.1016/j.fertnstert.2016.09.036. Izumi, Gentaro| Koga, Kaori| Takamura, Masashi| Makabe, Tomoko| Nagai, Miwako| Urata, Yoko| Harada, Miyuki| Hirata, Tetsuya| Hirota, Yasushi| Fujii, Tomoyuki| Osuga, Yutaka Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo, Tokyo, Japan.| Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo, Tokyo, Japan. Electronic address: kawotan-tky@umin.ac.jp.| Department OBJECTIVES: To characterize peritoneal dendritic cells (DCs) in endometriosis and to clarify their role in its etiology. DESIGN: Experimental. SETTING: University hospital. PATIENT(S): Sixty-three women (35 patients with endometriosis and 28 control women) who had undergone laparoscopic surgery. INTERVENTION(S): Peritoneal DCs from endometriosis and control samples were analyzed for the expression of cell surface markers. Monocyte-derived dendritic cells (Mo-DCs) were cultured with dead endometrial stromal cells (dESCs) to investigate changes in phagocytic activity and cytokine expression. MAIN OUTCOME MEASURE(S): Cell surface markers and cytokine expression and identification with the use of flow cytometry or reverse-transcription polymerase chain reaction (RT-PCR). Changes in cytokine expression and phagocytic activity of Mo-DCs cultured with dESCs and d-mannan were measured with the use of flow cytometry and RT-PCR. RESULT(S): The proportion of mannose receptor (MR)-positive myeloid DC type 1 was higher in endometriosis samples than in control samples. The blocking of MR reduced phagocytosis of dESCs by Mo-DCs. Mo-DCs cultured with dESCs expressed higher levels of interleukin (IL) 1beta and IL-6 than control samples. CONCLUSION(S): Peritoneal DCs in endometriosis tissue express high levels of MR, which promotes phagocytosis of dead endometrial cells and thereby contributes to the etiology of endometriosis. Mesh Terms: Case-Control Studies| Cells, Cultured| Coculture Techniques| Dendritic Cells/drug effects/immunology/*metabolism| Endometriosis/diagnosis/immunology/*metabolism/pathology| Female| Hospitals, University| Humans| Inflammation Mediators/metabolism| |
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