Results
| PMID | 28345488 |
| Gene Name | MSTN |
| Condition | Endometriosis (Deep infiltrating, ovarian endometriosis) |
| Association |
Associated |
| Sex | Female |
| Associated genes | ALK5, ActRIIB |
| Other associated phenotypes |
Endometriosis, endometrial cancer |
|
Reprod Sci. 2017 Dec;24(12):1577-1582. doi: 10.1177/1933719117698579. Epub 2017 Carrarelli, Patrizia| Funghi, Lucia| Ciarmela, Pasquapina| Centini, Gabriele| Reis, Fernando M| Dela Cruz, Cynthia| Mattei, Alberto| Vannuccini, Silvia| Petraglia, Felice 1 Department of Molecular and Developmental Medicine, Obstetrics and Gynecology, University of Siena, Siena, Italy.| 1 Department of Molecular and Developmental Medicine, Obstetrics and Gynecology, University of Siena, Siena, Italy.| 2 Department of Myostatin is a growth factor member of the transforming growth factor beta superfamily, which is known to play major roles in cell proliferation and differentiation. The present study investigated the messenger RNA (mRNA) expression of myostatin and myostatin receptors (activin receptor-like kinase 4 [ALK4], transforming growth factor (TGF)-beta type I receptor kinase [ALK5] and activin receptor type IIB [ActRIIB]) in endometrium of healthy women during menstrual cycle as well as in benign (endometriosis, polyps) and malignant (endometrial adenocarcinoma) conditions. Endometrial specimens were collected by hysteroscopy, whereas endometriotic lesions were collected by laparoscopy, and adenocarcinomas were sampled after hysterectomy. Total RNA was extracted from tissue homogenates, and gene expression was assessed by quantitative real-time polymerase chain reaction. Myostatin and myostatin receptors mRNAs were expressed by healthy endometrium throughout the menstrual cycle, with no differences between the proliferative and secretory phase. The highest myostatin mRNA expression was found in patients with deep infiltrating endometriosis (DIE) and in endometrial carcinoma; expression was also found in ovarian endometrioma (OMA ) and endometrial polyps. Myostatin receptors mRNA expression was higher in DIE and adenocarcinomas compared to control endometrium. The expression of ALK5 and ActRIIB in OMA was higher than in controls, whereas polyps had an increased expression of ALK5 mRNA. In conclusion, the present data showed for the first time the expression of myostatin in healthy endometrium and a higher expression in endometriosis and endometrial cancer, suggesting myostatin involvement in human endometrial physiology and related pathologies. Mesh Terms: Activin Receptors, Type I/metabolism| Activin Receptors, Type II/metabolism| Adult| Aged| Carcinoma, Endometrioid/*metabolism| Endometrial Neoplasms/*metabolism| Endometriosis/*metabolism| Female| Gene Expression| Humans| Menstrual Cycle| Middl |
|