Results
| PMID | 28856757 |
| Gene Name | CCL19 |
| Condition | Endometriosis |
| Association |
Associated |
| Population size | 70 |
| Population details | 70 (38 women with endometriosis, 32 controls) |
| Sex | Female |
| Associated genes | CCL19,CCR7 |
| Other associated phenotypes |
Endometriosis |
|
Am J Reprod Immunol. 2017 Nov;78(5). doi: 10.1111/aji.12744. Epub 2017 Aug 30. Diao, Ruiying| Wei, Weixia| Zhao, Jinghui| Tian, Fuying| Cai, Xueyong| Duan, Yong-Gang Centre of Reproductive Medicine, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, China.| Department of Obstetrics and Gynecology, Peking University Shenzhen Hospital, Shenzhen, China.| Department of O PROBLEM: The level of CCL19 increased in the peritoneal fluid of women with endometriosis, but the precise mechanism of CCL19/CCR7 in the pathogenesis of endometriosis remains unknown. METHODS: ELISA and immunohistochemistry were performed to analyze CCL19/CCR7 expressions in peritoneal fluid and endometrium from women with endometriosis (n = 38) and controls (n = 32). Cell proliferation and transwell invasion assays were applied to detect proliferation and invasion of human endometrial stromal cells (ESCs). Expressions of Bcl2, MMP2, MMP9, and p-AKT/AKT were analyzed by Western blot. RESULTS: Peritoneal fluid concentration of CCL19 in patients with endometriosis was higher than that in controls. Those patients with moderate/severe endometriosis had significantly higher peritoneal fluid concentrations of CCL19 compared to those with minimal/mild endometriosis. Higher CCL19 and CCR7 were found in the endometrium with endometriosis compared to control. CCL19 significantly enhanced ESC proliferation and invasion through CCR7 via activating PI3K/Akt signal pathways. CCL19/CCR7 interaction significantly enhanced phosphorylation of Akt, Bcl2, MMP2, and MMP9 in ESCs. CONCLUSION: These data indicate CCL19/CCR7 contributes to proliferation and invasion of ESCs, which are conducive to the pathogenesis of endometriosis through activating PI3K/Akt pathway. Mesh Terms: Adult| Cell Differentiation| Cell Movement| Cell Proliferation| Cells, Cultured| Chemokine CCL19/*metabolism| Embryonic Stem Cells/*physiology| Endometriosis/*immunology| Female| Humans| Oncogene Protein v-akt/metabolism| Phosphatidylinositol 3 |
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